P. B. Seshagiri

Molecular Reproduction, Development & Genetics
Research Areas: 
Early mammalian development and stem cell differentiation.
Research Highlights: 

Our group is interested in studying early mammalian development with regard to the acquisition of fertilizing potential of spermatozoa and development of embryos through peri-implantation stages with particular reference to the phenomenon of blastocyst hatching. Besides, we are interested in studying cell lineage specification by using ES-cells as model system. These studies contribute to our knowledge on the basic biology of early mammalian development, cellular differentiation and have implications in animal embryo biotechnology and in the management of human infertility and health. Looking at sperm biology, we are studying intra-spermatozoal cell signaling events, changes in sperm protein phosphorylation, particularly tyrosine phosphorylation signaling mechanism and their regulation during sperm function. We are studying ultrastructural localization and molecular characterization of tyrosine phosphorylated proteins and functionally, how they regulate sperm motility, required for fertilization competence.In the area of embryo biology, we are studying the cellular and molecular regulation of the critical peri-implantation developmental phenomenon and the bi-directional signaling mechanisms involving embryo-endometrial tissues, which engage a complex and a coordinated interplay of several growth factors (EGF, HB-EGF, LIF), transcription factors (Cdx-2, Oct-4), and proteases (cathepsins). The effects of these and other molecules during early development, especially during the period of blastocyst hatching are being investigated. Looking at stem cell biology, We intend to use wild-type and enhanced green fluorescent protein (EGFP)-expressing mouse ES-cells to understand cell lineage-specific differentiation and for experimental cell transplantation studies.