K. Muniyappa

Professor
Department of Biochemistry
Research Areas: 
Understanding the molecular mechanisms underlying chromosome synapsis, homologous recombination, double stranded break repair and Fanconi anemia - the hereditary chromosomal instability disorder.
Research Highlights: 

Our research focuses on understanding the molecular mechanisms underlying chromosome synapsis, homologous recombination, double stranded break repair and Fanconi anemia - the hereditary chromosomal instability disorder. Different experimental approaches currently are being used in the laboratory, which include genetics, functional and structural genomics, and model systems that range from bacteria to yeast to human cells. Fanconi anemia and Homologous Recombination (HR) - Fanconi anemia (FA) is an autosomal recessive and X-linked genetic disorder characterized by congenital abnormalities, progressive bone marrow failure and pronounced cancer susceptibility. Our research is aimed at elucidating the role of HR pathway components in FA cells. Synaptonemal complex (SC) proteins of Saccharomyces cerevisiae - Synapsis of homologous chromosomes and normal levels of recombination during meiosis require the participation of synaptonemal complex (SC). However, whether SC plays an active or a scaffolding role has been the subject of ongoing debate. We are studying yeast HOP1 (for HOmolog Pairing), which encodes a component of SC, to understand the mechanisms of interstitial synapsis of homologous chromosomes and recombination. DNA Double Strand Break Repair (DSBR) of Saccharomyces cerevisiae - DNA Double Strand Breaks (DSBs) are the most deleterious forms of DNA lesions. If left unrepaired, they lead to cell death. If repaired improperly, they can lead to chromosome translocations and cancer. Our research focuses on reconstituting the double-stranded end resection pathway of Saccharomyces cerevisiae. Additionally, we are trying to explore the role of homologous recombination proteins in the maintenance of genome integrity in Saccharomyces cerevisiae. Mechanistic aspects of homologous pairing and strand exchange - We are trying to gain insights into the mechanistic aspects of recombination and to elucidate the basis for inefficient allele exchange in the Tuberculosis bacillus. Our interest in this area will expand to include comparative genomics to understand the phylogenetic relationship between pathogenic and non-pathogenic species of mycobacteria. It is hoped that the knowledge gained from these studies will enable us to reconstitute the pathway of homologous recombination in mycobacteria, which may further help in the molecular genetic manipulation of this class of bacteria.

Phone: 
91-80-22932235