Central Animal Facility

The Small Animal Facility was built in 1991 with a total area of 1680 sft for breeding of laboratory animals and few additional rooms for supporting activities. Due to increased usage of animals by the investigators and also demand for more number of rooms for animal experimentation, additional two floors were added in 1996 with a total area of 2520 sft. The second floor was built with clean air concept to carry out research on transgenic and mutant strains of rodents.

The Small Animal Facility breeds and maintains genetically pure inbred strains of different species of animals for research activities. The animal species includes rats (Wistar, Sprague Dawley), mice (BALB/c, C57BL/6, Swiss albino, FVB/N, CD1, C3HeJ, Nude, Knockouts (IFN KO, INoS KO, etc.) and rabbit (New Zealand white) (Table 1). The research work involving animals is carried out in all the departments (Biochemistry, Microbiology and Cell Biology, Molecular Reproduction and Developmental Genetics, Molecular Biophysics Unit, Centre for Neuro Sciences) of Division of Biological Sciences, Dept. of Mechanical Engineering, Material Research Centre, Materials Engineering, Inorganic and Physical Chemistry, and Centre for Nano Science and Engineering at the Indian Institute of Science (Fig 1). Currently 66 faculty members are using the animal facility and the list of faculty members who are using Central Animal Facility is given in Table 2. Approximately 160 research workers which include post-doctoral fellows, research associates, and graduate students use this facility on a regular basis every year. The research work is carried out in various disciplines of science which includes biochemistry, microbiology, reproductive biology, cell biology, immunology, virology, cancer biology, developmental biology etc. The Small Animal Facility supplied 10,800 animals in 2012 (Jan-Dec) and 11,300 animals in 2013 (Jan-Dec). A total of 55 project proposals in 2012 and 26 project proposals on animals were approved by the Institution Animal Ethics Committee (IAEC) in 2013. A total of 53 papers were published in 2012 (Jan – Dec) and 52 papers were published in 2013 (Jan - Dec) by using animals from Central Animal Facility.

Currently CAF is maintaining eight strains of wild type of mice and ten different strains of mutant mice. Recently we procured another 10 strains of mutant (knockout/transgenic) mice. A clean air facility (~900 sft.) was established (Fig. 2) in the second floor of CIDR complex to carryout research with BSL1 and BSL2 organisms.

Table 1: List of animals that are bred and maintained at CAF

List of wild type mice strains

BALB/cJ

General purpose mouse strains and are being used commonly for routine research activities by most of the investigators.

 

 

 

 

 

BALB/cCAF

BALB/cNIV

C57BL/6J

C3He/J

Swiss albino

CD1

List of rat strains

Wistar

General purpose rat strains and are being used for routine research work.

Sprague Dawley

Rabbit strain

New Zealand White

Used for production of polyclonal antibodies

List of gene knockout/null Mice

B6.129S7-Ifngtm1ts/J

Mice homozygous for the Ifngtm1Ts targeted mutation and are useful in studies of macrophages and expression of MHC.

B6.129P2-Nos2tm1Lau/J

Mice homozygous for Nos2 (nitric oxide synthase 2, inducible, macrophage) targeted mutation is useful for studying wound healing, inflammatory bowel disease, immune response to infection, tumorigenesis and apoptosis.

C57BL/6-Tg(TcraTcrb)1100Mjb/J

These mice contain transgenic inserts for mouse Tcra-V2 and Tcrb-V5 genes. They are useful to study the role of peptides in positive selection and the response of CD8+ T cells to antigen.

B6.129S6-Cybbtm1Din/J

These mice are with a null allele of the gene involved in Chronic granulomatous disease (CGD. This animal model aid in developing new treatments for CGD and in evaluating the role of phagocyte-derived oxidants in inflammation.

B6.129-Tlr2tm1Kir/J

Homozygotes for the Tlr2tm1Kir mutation. This strain is useful for studying responses to bacterial endotoxins.

FVB-Tg(GFAP-cre)25Mes/J

This transgenic mouse strain expresses Cre recombinase under the control of the human glial fibrillary acidic protein promoter (GFAP). Used for Glioma studies

Nude HSD-Fox N1

Immunocompromised strain and are used in oncology, transplantation, tumor cell growth, immunology and autoimmune disease studies.

NOD.CB17-Prkdcscid/J

B6.MRL-Faslpr/J

Mice homozygous for the lymphoproliferation spontaneous mutation (Faslpr) show systemic autoimmunity. This mouse is a model for systemic lupus erythematosus-like autoimmune syndromes.

B6(Cg)-Ncf1m1J/J

Used in understanding the mechanisms of phagocytosis.

SRF floxed mice

Used in understanding neurobiological mechanisms

Vinculin floxed mice

Med1 floxed mice

GFAP-Cre transgenic mice

Nestin-Cre transgenic mice

CamKII-a Cre transgenic mice

GFAP-CreERT2-Cre transgenic mice

SRF floxed mouse carrying GFAP or Nestin Cre

Vinculin floxed mouse carrying NEX-Cre or Nestin-Cre

Med1 floxed mice carrying CaMKII-a Cre

 

Table 2: List of faculty members who are using Small Animal Facility


Sl.#

Name

Dept.

Sl.#

 

Dept.

1

Prof. Anjali A. Karande

BC

34

Prof. Ajitkumar

MCB

2

Prof. Dipankar Nandi

BC

35

Prof. Balaji K.N.

MCB

3

Dr. Ganesh Nagaraju

BC

36

Prof. Dipshikha Chakravortty

MCB

4

Prof. Jayabhaskaran C.

BC

37

Prof. Durga Rao C.

MCB

5

Prof. Manjunath R.

BC

38

Prof. Kumar Somasundaram

MCB

6

Prof. Muniyappa K.

BC

39

Prof. Nagaraja V.

MCB

7

Prof. Padmanabhan G.

BC

40

Dr. Saibal Chatterjee

MCB

8

Dr. Patrick D’Silva

BC

41

Prof. Shaila M.S.

MCB

9

Prof. Rangarajan P.N.

BC

42

Dr. Subba Rao G.

MCB

10

Prof. Rao A.J.

BC

43

Prof. Saumitra Das

MCB

11

Prof. Rao D.N.

BC

44

Prof. Umesh Varshney

MCB

12

Prof. Sathees Raghavan C.

BC

45

Prof. Utpal Nath

MCB

13

Prof. Savithri H.S.

BC

46

Prof. Usha Vijayaraghavan

MCB

14

Prof. Utpal Tatu

BC

47

Prof. Vijaya S.

MCB

15

Dr. Arun Nagaraj

BC

48

Dr. William Rasican Surin

MCB

16

Dr. Ramachandra S.G.

CAF

49

Dr. Ravi Sundaresan

MCB

17

Dr. Ravindranath H. A.

CAF

50

Prof. Annapoorni Rangarajan

MRDG

18

Dr. Balaji Jayaprakash

CNS

51

Prof. Arun Kumar

MRDG

19

Prof. Aditya Murthy

CNS

52

Prof. Kondaiah P.

MRDG

20

Dr. Arun Sripathi

CNS

53

Prof. Medhamurthy R.

MRDG

21

Dr. Deepak Nair

CNS

54

Prof. Nanjundaiah V.

MRDG

22

Dr. Supratim Ray

CNS

55

Prof. Rajan R Dighe

MRDG

23

Prof. Naren Ramanan

CNS

56

Prof. S. Visweswariah

MRDG

24

Prof. Shyamala Mani

CNS

57

Prof. Seshagiri P.B.

MRDG

25

Prof. V. Ravindranath

CNS

58

Dr. Upendra N

MRDG

26

Prof. Dipankar Chatterjee

MBU

59

Dr. Deepak Saini

MRDG

27

Prof. Gopal B.

MBU

60

Prof. Bikramjit Basu

MRC

28

Dr. Rishikesh Narayanan

MBU

61

Dr. Kaushik Chatterjee

Mat.Eng

29

Prof. Sikdar S.K.

MBU

62

Prof. Ashok M. Raichur

Mat.Eng

30

Prof. Suguna K.

MBU

63

Dr. Sandya S

IPC

31

Prof. Surolia A.

MBU

64

Dr. Samuelson

IPC

32

Prof. Varadarajan R.

MBU

65

Dr. Namrata Gundaiah

ME

33

Dr. Amit Singh

MCB

66

Dr. Ambarish Ghosh

CeNSE

 

 

 

 

Fig. 1 : Utilization of animal facility by various departments

 

 

Fig 2. View of clean air facility

 

 

Work in progress:

  1. Currently, a barrier facility (Class 100,000) of approximately 3500 sft is under construction in the second floor of CIDR complex with controlled environmental conditions for animal experimentation. It will be ready for use in another two months.

  1. Efforts are ongoing towards establishment new animal facility adjacent to the existing animal facility. This facility will be a three floor building with a total area of approximately 36,000 sft. The facility will be built as per the international standards with two corridor system concept in the second floor and single corridor system in ground and first floor. The facility will have dedicated air handling units and will be built as per Class 100,000 (ISO 8) (separate AHU for each floor). Ground and first floor will be used for non-infectious studies and second floor will be used exclusively for infectious studies.